The University of Louisville is a state supported urban university located in Kentucky's largest metropolitan area. It was a municipally supported public institution for many decades prior to joining the university system in 1970. The University has three campuses.

The Southern Baptist Theological Seminary is the flagship seminary of the Southern Baptist Convention.

Founded in 1814, Spalding University is a co-educational urban university in the Catholic tradition for students of all faiths. Its name honors Catherine Spalding, foundress of the Sisters of Charity of Nazareth. Innovative, the University today utilizes the City of Louisville as a laboratory for its academic programs, many of which are unique in the area and in the Commonwealth.

Located in Louisville, KY and Pittsburgh, PA RETS Institute of technology is a career college that provides certificat, degree, and diploma programs in MCSE, Information Technology, Network Administration, and Computer Programming.